<p class="MsoNormal" align="center" style="margin-top:7.8000pt;margin-bottom:7.8000pt;mso-para-margin-top:0.5000gd; mso-para-margin-bottom:0.5000gd;text-align:center;"><b><span style="font-family: 隶书; font-size: 22pt;">卡那霉素酶联免疫反应试剂盒</span></b><b><span style="font-family: 隶书; font-size: 22pt;"><o:p></o:p></span></b></p><p class="MsoNormal" align="center" style="margin-top:7.8000pt;margin-bottom:7.8000pt;mso-para-margin-top:0.5000gd; mso-para-margin-bottom:0.5000gd;text-align:center;"><b><span style="font-family: 隶书; color: rgb(0, 0, 255); font-size: 22pt;">Ounuo</span></b><b><span style="font-family: 隶书; font-size: 22pt;">990</span></b><b><span style="font-family: 隶书; font-size: 22pt;">01</span></b><b><span style="font-family: 隶书; font-size: 22pt;"><o:p></o:p></span></b></p><p class="MsoNormal"><b>1.概述</b><b><o:p></o:p></b></p><p class="MsoNormal" style="margin-left:8.9000pt;mso-para-margin-left:0.8500gd;text-indent:18.0000pt; mso-char-indent-count:2.0000;"><span style="font-size: 9pt;">卡那霉素酶联免疫反应测试盒是用于</span><span style="font-size: 9pt;">肾脏、肝脏、肌肉（牛肉、鸡肉和猪肉）、细胞裂解液、血、尿、牛奶</span><span style="font-size: 9pt;">中卡那霉素残留的定量检测。</span><span style="font-size: 9pt;">该试剂盒有以下</span><span style="font-size: 9pt;">特点</span><span style="font-size: 9pt;">：</span><b><o:p></o:p></b></p><p class="MsoNormal" style="margin-left:42.0000pt;text-indent:-21.0000pt;mso-layout-grid-align:none; text-autospace:none;mso-list:l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family:Wingdings;mso-fareast-font-family:宋体;mso-bidi-font-family:Arial; font-size:9.0000pt;mso-font-kerning:0.0000pt;">&#216; </span><!--[endif]--><span style="font-size: 9pt;"><font face="宋体">快速</font><font face="宋体">(10-40分钟)，多种样品的非有机溶剂的提取方法，高回收率(>80</font></span><span style="font-size: 9pt;">%</span><span style="font-size: 9pt;">)</span><span style="font-size: 9pt;">。</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left:42.0000pt;text-indent:-21.0000pt;mso-layout-grid-align:none; text-autospace:none;mso-list:l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family:Wingdings;mso-fareast-font-family:宋体;mso-bidi-font-family:Arial; font-size:9.0000pt;mso-font-kerning:0.0000pt;">&#216; </span><!--[endif]--><span style="font-size: 9pt;">高灵敏度（</span><span style="font-size: 9pt;">0.2</span><span style="font-size: 9pt;">ppb）。</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left:42.0000pt;text-indent:-21.0000pt;mso-layout-grid-align:none; text-autospace:none;mso-list:l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family:Wingdings;mso-fareast-font-family:宋体;mso-bidi-font-family:Arial; font-size:9.0000pt;mso-font-kerning:0.0000pt;">&#216; </span><!--[endif]--><span style="font-size: 9pt;">高重复性。</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left:42.0000pt;text-indent:-21.0000pt;mso-layout-grid-align:none; text-autospace:none;mso-list:l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family:Wingdings;mso-fareast-font-family:宋体;mso-bidi-font-family:Arial; font-size:9.0000pt;mso-font-kerning:0.0000pt;">&#216; </span><!--[endif]--><span style="font-size: 9pt;"><font face="宋体">快速的</font>ELISA检测方法（</span><span style="font-size: 9pt;">不考虑样本量的前提下</span><span style="font-size: 9pt;">只需不到</span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">小时）。</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left:21.0000pt;mso-layout-grid-align:none;text-autospace:none;"><span style="font-size: 9pt;"> </span></p><p class="MsoNormal"><b>2</b><b>.试剂盒</b><b>原理</b><b><o:p></o:p></b></p><p class="MsoNormal" style="margin-left:8.9000pt;mso-para-margin-left:0.8500gd;"><span style="font-size: 9pt;">卡那霉素</span><span style="font-size: 9pt;">酶联免疫反应测试盒基于竞争性酶联反应原理，抗原已经包被于微孔板上。分析时，样品、酶标物以及抗体加入孔中，如果样品中含有</span><span style="font-size: 9pt;">药物</span><span style="font-size: 9pt;"><font face="宋体">，会与微孔板上的抗原竞争抗体，同时酶标物与抗体结合。加入</font><font face="宋体">TMB底物，底物的颜色显色强度与样品中</font></span><span style="font-size: 9pt;">卡那霉素</span><span style="font-size: 9pt;">的含量成反比。</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left:8.9000pt;mso-para-margin-left:0.8500gd;"><span style="font-size: 9pt;"> </span></p><p class="MsoNormal"><b>3</b><b>.</b><b>试剂盒组成</b><b><o:p></o:p></b></p><p class="MsoNormal" style="text-indent: 27pt;"><span style="font-size: 9pt;"><font face="宋体">已包被抗原的酶标板</font><font face="宋体">(Kanamycin-coated Plate)（可拆式）</font></span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1×96孔</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">标准液</span><span style="font-size: 9pt;">(Standards)</span><span style="font-size: 9pt;"><font face="宋体">（</font>0，</span><span style="font-size: 9pt;">0.2</span><span style="font-size: 9pt;">，</span><span style="font-size: 9pt;">0.6</span><span style="font-size: 9pt;">，</span><span style="font-size: 9pt;">1.8</span><span style="font-size: 9pt;">，</span><span style="font-size: 9pt;">5.4</span><span style="font-size: 9pt;">，</span><span style="font-size: 9pt;">16.2</span><span style="font-size: 9pt;">ppb）</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">6</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">1.0</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">高标液</span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">000</span><span style="font-size: 9pt;">ppb（Spiking）</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1×</span><span style="font-size: 9pt;">1.0</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">一抗</span><span style="font-size: 9pt;">(Antibody #1)</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">6.0m</span><span style="font-size: 9pt;">L</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">HRP酶标</span><span style="font-size: 9pt;">二抗</span><span style="font-size: 9pt;">(HRP-Conjugated </span><span style="font-size: 9pt;">Ab#2</span><span style="font-size: 9pt;">)</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">6.0m</span><span style="font-size: 9pt;">L</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">10</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">样品稀释</span><span style="font-size: 9pt;">液</span><span style="font-size: 9pt;">（</span><span style="font-size: 9pt;"><font face="Arial">10</font><font face="宋体">×</font></span><span style="font-size: 9pt;">Sample diluent）</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">15</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">20</span><span style="font-size: 9pt;">×浓缩</span><span style="font-size: 9pt;">洗液</span><span style="font-size: 9pt;">(Wash Solution)</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">28</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 24.45pt;"><span style="font-size: 9pt;">TMB</span><span style="font-size: 9pt;">底物</span><span style="font-size: 9pt;">(TMB Substrate)</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">1</span><span style="font-size: 9pt;">2</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="text-indent: 27pt;"><span style="font-size: 9pt;"><font face="宋体">终止液</font><font face="宋体">(Stop Buffer)</font></span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1×1</span><span style="font-size: 9pt;">2</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 27pt;"><span style="font-size: 9pt;">10</span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">样品提取</span><span style="font-size: 9pt;">液</span><span style="font-size: 9pt;">(</span><span style="font-size: 9pt;"><font face="Arial">1</font></span><span style="font-size: 9pt;"><font face="Arial">0</font></span><span style="font-size: 9pt;">×</span><span style="font-size: 9pt;">Sample Ext. Buffer)（</span><span style="font-size: 9pt;">可</span><span style="font-size: 9pt;">选）</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1×</span><span style="font-size: 9pt;">15</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal" style="margin-left: 27pt;"><span style="font-size: 9pt;"><font face="宋体">样品平衡液（</font><font face="宋体">Sample Balance buffer）</font></span><span style="font-size: 9pt;">（</span><span style="font-size: 9pt;">可</span><span style="font-size: 9pt;">选）</span><span style="font-size: 9pt;"> </span><span style="font-size: 9pt;">1×</span><span style="font-size: 9pt;">2.0</span><span style="font-size: 9pt;">mL</span><span style="font-size: 9pt;"><o:p></o:p></span></p><p class="MsoNormal"><span style="font-size: 9pt;"><font face="宋体">本试剂盒应当在</font>2-8℃的温度下储存，有效期为一年。</span><b><span style="font-size: 9pt;"><font face="宋体">如果超过</font><font face="宋体">1个月不使用试剂盒，请</font></span></b><b><o:p></o:p></b></p><p class="MsoNormal"><b><span style="font-size: 9pt;"><font face="宋体">将标品、一抗和二抗放置</font><font face="宋体">-2</font></span></b><b>0</b><b>℃或者冰冻保存。</b><b><o:p></o:p></b></p><p class="MsoNormal"><b> </b></p><p class="MsoNormal"><b>4.</b><b>检测限</b><b><o:p></o:p></b></p><div align="center"><table class="MsoNormalTable" border="1" cellspacing="0" style="border-collapse:collapse;mso-table-layout-alt:fixed;border:none; mso-border-left-alt:0.5000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;mso-border-insideh:0.5000pt solid windowtext;mso-border-insidev:0.5000pt solid windowtext; mso-padding-alt:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;"><tbody><tr><td width="285" valign="top" style="width:171.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:1.0000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;"><p class="MsoNormal" align="center" style="text-align:center;"><b>检测物质</b><b><o:p></o:p></b></p></td><td width="214" valign="top" style="width:128.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:1.0000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;"><p class="MsoNormal" align="center" style="text-align:center;"><b><font face="宋体">检测下限（</font>ng/g）</b><b><o:p></o:p></b></p></td></tr><tr><td width="285" valign="top" style="width:171.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-size: 9pt;">鱼</span><span style="font-size: 9pt;">/</span><span style="font-size: 9pt;">虾</span><span style="font-size: 9pt;">/</span><span style="font-size: 9pt;"><font face="宋体">肉类</font><font face="宋体">/肝/肾</font></span></p></td></tr></tbody></table></div>